After the death of his wife in a mass shooting, secret agent Jason Bourne is convinced that there is more to her murder than it seems. Worse, he believes that the agency that trained him and made him who he is--Treadstone--is behind the killing. Bourne goes rogue, leaving Treadstone behind and taking on a new mission to infiltrate and expose an anarchist group, Medusa.

But when a congresswoman is assassinated in New York, Bourne is framed for the crime, and he finds himself alone and on the run, hunted by both Treadstone and the tech cabal who had hired him. In his quest to stay one step ahead of his enemies, Bourne teams up with a journalist, Abbey Laurent, to figure out who was behind the frame-up, and to learn as much as he can about the ever-growing threat of the mysterious Medusa group. As more and more enemies begin to hunt Bourne, it's a race against the clock to discover who led him into a trap...and what their next move may be.